Online Raman for Process Real-time analysis of multiple components in fermentation broth

JINSP RS2000/RS2000-4 online Raman Process analyzer
In-situ, continuous online monitoring of fermentation/culture broth with real-time feeding feedback enables precise process control.

• Real time feeding feedback for fermentation engineering and cell engineering;
• Simultaneous monitoring of multiple components including raw materials, OD values, and products, with real-time feedback for auxiliary adjustment.

Biological fermentation engineering is one of the important components of modern biopharmaceutical engineering, which obtains the required biochemical products through the growth process of microorganisms. The growth process of microorganisms includes four stages: adjustment period, logarithmic period, stable period, and decay period. During the stable phase, a large amount of metabolic products accumulate, which is also the period when most reactions harvest products. Once this period is crossed and enters the decay phase, both bacterial activity and product purity will be greatly affected. Due to the complexity of biological reactions, poor repeatability of fermentation processes, and difficulty in quality control, abnormal reactions are prone to occur during the process from laboratory to pilot scale, and from pilot scale to large-scale production. How to ensure that fermentation reactions are maintained in a stable period for a long time is a concern for the scaling up of fermentation engineering processes.

It is crucial to maintain the content of raw materials, OD values, and products in order to ensure that the bacterial strains are in a vigorous and stable growth state during the fermentation process. Using online spectroscopy to monitor the above indicators of fermentation broth in real time is a technical route suitable for controlling the biological fermentation process: using raw materials, OD values, and product changes as indicators for feeding and determining the state of bacterial strains, and adjusting feeding in a timely manner when the content is abnormal, can significantly improve the product quality and production efficiency of biological fermentation. As shown in the figure below, an immersion optical probe is inserted on the side of a small fermentation tank to obtain real-time fermentation liquid signals, and ultimately monitor the raw materials, OD values, and products simultaneously.

If offline sampling and laboratory testing of fermentation broth are used for process control, on the one hand, delayed detection results may miss the opportunity for feeding intervention, and on the other hand, the sampling process may have an impact on the fermentation system, such as contamination by miscellaneous bacteria.